Composite

Part:BBa_K5301026:Design

Designed by: Ruiyao Xu   Group: iGEM24_BNU-China   (2024-09-25)


sGFP1-10 tether expression pathway (T7)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 159
    Illegal AgeI site found at 219
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The T7 expression system is used to express this pathway.


Source

The T7 promoter sequence comes from BBa_K2406020, the mSA sequence comes from BBa_K4623001, the sGFP1-10 sequence comes from BBa_K5301012, and the T7 terminator sequence comes from BBa_B0012.

References